Heny Ekowati*, Amelia Meisya Anwar, Fuzilestari Nur Audina, Esti Dyah Utami

Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University

Jl. Dr. Soeparno, Karangwangkal, Purwokerto, Central Java, Indonesia, 53123

*Corresponding author, phone. +62 812 31984072, email: heny.ekowati@unsoed.ac.id

Co-author-1, email: amaliameisyaa@gmail.com

Co-author-2, email: fuziaudina@gmail.com

Co-author-3, email: estinesia@gmail.com

ABSTRACT

Introduction: Curcuma longa (CL) and Alpinia galanga (AG) have the potential gastroprotective activity. In this study, we evaluated the gastroprotective effects of C. longa and A. galanga extract against gastric mucosal lesions induced by ethanol-hydrochloric acid (HCl) in rat model.

Method: The experimental design comprised five groups of rats, each group consist of 4 rats. Group I (normal control group) received tween 80 (1%), group II (positive control) was administered ranitidine (50 mg/kgBW) orally. The other three groups received CL, AG and combination (1:1) (100 mg/200kgBW) orally. All groups were treated for 6 days. On the 7^th day, all groups were induced by ethanol-HCl (1: 1) 0.2 ml/25 gBW. Four hours later the rat was sacrificed and dissected for gastric. Gross ulcer urea, lesion length, histology (H & E staining), pH and ulcer-index were assessed to determine the potentiality of extract in prevention against gastric ulcers.

Results: Histopathological profile showed ulcer repairment and reduction of neutrophil inflammatory cell infiltration in treatment group. There were also improvement in pH, lesion score, ulcer index, and curative ratio. However, there were no significant differences between single and combination extracts.

Conclusions: C. longa, A. galanga, and combination extract have gastroprotective effect in rat model.

Keywords: Alpinia galanga, Curcuma longa, gastroprotective, rats.