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Background: Bitter herbs (Andrographis paniculata Ness.) is used for various treatments, including antioxidants, diabetes mellitus, and anticancer. Flavonoid is one of the dominant compounds in bitter herbs. Purpose: This study aims to identify secondary metabolites contained in bitter herbs. Methods: Extraction of bitter herbs was carried out by maceration method using ethanol as a solvent. The extracts were partitioned successively using n-hexane, chloroform, and ethyl acetate. The results of practitioners then identified the most dominant flavonoid content. This fraction was selected for column chromatography. Results: The ethanolic extraction of bitter herbs was obtained at a yield of 12.35%. Partitioning was carried out using a separating funnel to obtain the n-hexane fraction, chloroform fraction, ethyl acetate fraction and residue. The ethyl acetate fraction has the most dominant flavonoid content. The ethyl acetate fraction was fractionated by column chromatography. Identification of sub-fraction 6 was carried out using TLC and citroborate staining. Conclusion: The results of the identification of bitter herbs obtained flavonoid compounds of the flavonol group.