Fusarium wilt disease is a major constraint in the production of bananas. The disease is caused by Fusarium oxysporum f. sp cubense (Foc), a soil-borne pathogen that has a wide host range. This study aims to determine the molecular characteristic of F. oxysporum from taro and its pathogenicity in four banana cultivars. Molecular characterization of F. oxysporum was conducted at the Laboratory of Plant Protection Unsoed. The in-planta test consisted of two steps. The first step was a selection for inoculation techniques, carried out by inoculation of Foc isolates from taro to healthy taro using the pour and stick method. As a result, the pour method infected faster than the stick method showed yellowing symptoms on the third day. The second step was a pathogenicity test of F. oxysporum on the banana seed of Cavendish, Mas, Raja, and Kepok cultivars using the pour method. The result indicated that F. oxysporum from taro has the same characteristic as Foc, and was pathogenic on the plant test. Infected bananas showed yellowing symptoms on the day sixth, starting from the old leaves, followed by wilt and die.