UNSOED Conferences, The 3rd International Conference On Sustainable Agriculture For Rural Development (3rd ICSARD)

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Phytochemical Characteristic and Antimicrobial Activity of Coconut Husk Extract
Karseno Karseno

Last modified: 2023-02-16

Abstract


Coconut husk is a by-product of the coconut that known contains phytochemical compounds and antimicrobial activities. This study aims to determine the phytochemical characteristics and the antimicrobial activity of coconut husk extracts. Completely Randomized Design was used in this study and the effect of maturity level of coconut husk and the solvent type were investigated. The maturity level of coconut husk consists of young and old coconut husk, and the solvent type were aquadest, ethanol, ethyl acetate and n-hexane. The phytochemicals characteristic, pH and antimicrobial activity of coconut husk extract were investigated. The results of this study indicate that young coconut husk extract containing tannins, flavonoids and steroids. Meanwhile, the old coconut husk extract containing tannins, flavonoids, steroids and terpenoids. Both of young and old coconut husk extracts showed antimicrobial activity against S. cerevisiae, A. aceti and the microbes isolated from fermented sap. Old coconut husk extract show antimicrobial activity stronger than young coconut husk extract and the old coconut husk extracted with aquadest show the highest antimicrobial activity. This extract contains a total phenolic of 76.04 mg GAE/g, total flavonoid of 1.57 mg QE/g and total tannin of 522.95 mg TAE/g, respectively. In addition, based on the absorbance spectrum, it was confirmed that this extract contains tannins, flavonoids and steroids.