Spinach is one of the important horticultural crops because they are easy to grow and its nutritional value, especially Fe content, is higher than other vegetables. Red spinach has higher anthocyanin content than green spinach so it has a higher economic value. Mostly, they are cultivated hydroponically in Indonesia. In addition to using inorganic chemical compounds, they are also susceptible to root rot disease. Utilization of ecoenzymes made from vegetable and fruit waste as biopesticides and liquid organic fertilizers can be used as an alternative to avoid the use of synthetic chemical pesticides and inorganic fertilizers. This study aims to determine whether the ecoenzyme can be used to increase plant growth and health, especially against root rot disease in hydroponic vegetable cultivation. The research to be carried out is a two-factor study with three replications arranged at completely randomized groups. The first factor is inoculation and no inoculation of pathogens Phytophthora sp. while the second factor is the concentration of ecoenzymes consisting of 1000 ppm, 2000 ppm and 3000 ppm. The results showed that the application of ecoenzymes did not produce different growth results with controls both inoculated and without pathogen inoculation, while the incidence of disease decreased to 81.82% with the application of 3000 ppm ecoenzymes.